As a Young Offenders Institution, HMYOI Aylesbury holds long term male inmates between the ages of 18 and 21 only. Accommodation comprises single cells in seven residential wings and one segregation unit. Currently due to special measures the prison is only operating four of the seven residential units.

There are full and part-time education programmes at the YOI, which range from basic and key skills courses up to Art, French and Sociology at A Level and Physical Education. Vocational courses and work programmes include Construction, Painting and Decorating, Bricklaying, Motor Vehicle Mechanics, Industrial and General Cleaning, Laundry, Catering, Gardening and Waste management. Young Offenders also have opportunities to pursue award-based courses including the Duke of Edinburgh’s Award.